Localisation of Flamingo in different mutant backgrounds

Flamingo in a strabismus⁶ mutant clone

Loss of Strabismus leads to a reduction of flamingo from the cell junctions, and a loss of asymmetry.

Strabismus is required for junctional localisation of Flamingo.

The image shows the gene name.

Flamingo reduced at junctions in absence of Strabismus.

Reference

Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning. R Bastock, 2003

• Flybase reference

• Pubmed reference

Flamingo in a frizzled mutant background

Loss of frizzled disrupts Flamingo junctional localisation and asymmetry.

Flamingo levels are reduced and is no longer asymmetric in the absence of Frizzled.

Imunostaining for Flamingo in pupal wing cells mutant for frizzled^K21/frizzled^D21.

Reference

Flamingo, a seven-pass transmembrane cadherin, regulates planar cell polarity under the control of Frizzled. T Usui et al., 1999

• Flybase reference

• Pubmed reference

Flamingo in a dishevelled³ mutant background

Loss of dishevelled disrupts Flamingo junctional localisation and asymmetry.

Flamingo is no longer asymmetric in absence of Dishevelled.

This result was also published in Ressurreição et al. (2018) using dsh^V26.

Reference

Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning. R Bastock et al., 2003

Rapid disruption of Dishevelled activity uncovers an intercellular role in maintenance of Prickle in core planar polarity protein complexes. Ressurreição et al., 2018

Flamingo in a diego³⁸⁰ mutant background

Loss of diego disrupts Flamingo cellular asymmetry, but Fmi is still localised to the cell membrane.

Flamingo is no longer asymmetric in absence of Diego.

Flamingo is abnormally polarised in whorls.

"Flamingo sometimes polarises its distribution in parts of dgo380 mutant wings at later times (29 to 30 hours after puparium formation), but the axis of polarity is often abnormal." Feiguin et al., 2001

Reference

The Ankyrin repeat protein Diego mediates Frizzled-dependent planar polarization. F Feiguin, M Hannus, M Mlodzik, and S Eaton, 2001

• Flybase reference

• Pubmed reference

Flamingo in a prickle mutant background

Loss of pickle disrupts Flamingo cellular asymmetry.

Flamingo is no longer asymmetric in absence of Prickle

Imunostaining for Flamingo in pupal wing cells mutant for pk-sple¹³.

Reference

Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning. R Bastock, 2003

• Flybase reference

• Pubmed reference (Bastock et al.)

Flamingo in a prickle over-expression background

Addition of pickle disrupts Flamingo cellular asymmetry and increases the size of Fmi puncta.

Flamingo puncta increase in size when Prickle is over-expressed.

A Ptc-GAL4/UAS-Pk wing at compartment boundary (red denotes the Pk expression).

Reference

Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning. R Bastock, 2003

• Flybase reference

• Pubmed reference (Bastock et al.)

Flamingo in a Dishevelled over-expression background

Addition of Dishevelled disrupts Flamingo cellular asymmetry and increases the size of Flamingo puncta.

Flamingo puncta increase in size when Dishevelled is over-expressed.

Flamingo in a Diego over-expression background

Addition of Diego disrupts Flamingo cellular asymmetry and increases the size of Flamingo puncta.

Flamingo puncta increase in size when Diego is over-expressed.

Reference

The Ankyrin repeat protein Diego mediates Frizzled-dependent planar polarization. F Feiguin, M Hannus, M Mlodzik, and S Eaton, 2001, Dev cell

Flybase reference

Flamingo in a Fz over-expression background

Addition of Fz disrupts Flamingo cellular asymmetry.

Reference

• Developmental Cell reference

• Flybase reference

Flamingo in a Stbm over-expression background

Addition of Stbm disrupts Flamingo cellular asymmetry.

Reference

• Developmental Cell reference

• Flybase reference