Localisation of Diego in different mutant backgrounds

Diego in a flamingo^E59 clone

Loss of flamingo leads to loss of Diego from the junctions.

Flamingo is required for junctional localisation of Diego.

Confocal images of pupal wings at about 28 hours. Distal is in the top right hand corner Clone is mutant for flamingo^E59.

Reference

The ankyrin repeat protein Diego mediates Frizzled-dependent planar polarization. F Feiguin, M Hannus, M Mlodzik, S Eaton, 2001.

• Pubmed reference

• Flybase Diego reference

• Flybase paper reference

anti-Diego in a frizzled mutant background

Loss of frizzled disrupts Diego junctional localisation and asymmetry.

Diego reduced at junctions and not asymmetric in absence of Frizzled.

Confocal images of pupal wings at about 28 hours. Distal is top. Loss-of-function clones, mutant tissue indicated by absence of lacZ staining (red, left panel).

Reference

The ankyrin repeat protein Diego mediates Frizzled-dependent planar polarization. F Feiguin, M Hannus, M Mlodzik, S Eaton, 2001.

• Pubmed reference

• Flybase Diego reference

• Flybase paper reference

Diego in a dishevelled mutant background

Loss of Dishevelled reduces Diego levels (Wu 2008).

Loss of dishevelled disrupts Diego astymmetry.

Pupal eye image, arm-lacZ FRT18/dishevelled^v26 FRT18; hsFLP/+

Diego localisation in the pupal eyeDiego is reduced apically but still present, so Frizzled recruits Diego largely independently of Dishevelled -Wu et al. 2008.

This seems unlikely given the images shown. More likely that Dishevelled is required for Diego recruitment to the junctions and in the absence of Dishevelled remains in the cytosol. A wing image would give a clearer result.

Reference

Jun Wu, Andreas Jenny, Ivana Mirkovic, Marek Mlodzik, 2008.

• Pubmed reference

Diego in a Strabismus mutant background

Unpublished.

Loss of strabismus disrupts Diego astymmetry.

Diego in a prickle mutant background

Diego in a >>Prickle over-expression background

Overexpression of Prickle leads to a loss of Diego asymmetry.

Over expression of >>Dishevelled leads to a loss of Diego asymmetry

Over expression of Flamingo leads to a reorientation of Diego asymmetry and the formation of large puncta

Diego asymmetry is re-orientated to be in parallel to the Flamingo overexpressing cells.

Over expression of Flamingo leads to an increase in Diego.

Patch-Gal4 UAS-Flamingo in pupal wings.

Diego is enriched at distal sides of cells (Das et. al. 2004). The paper referred to here (Feiguin, 2001) suggests, without evidence, that Diego could be on both sides of the cell.

Reference

The ankyrin repeat protein Diego mediates Frizzled-dependent planar polarization. F Feiguin, M Hannus, M Mlodzik, S Eaton, 2001.

• Flybase reference

• Pubmed reference

Dishevelled when >>Frizzled is overexpressed

Over expression of Frizzled leads to a loss of Diego asymmetry.

Dishevelled when >>Strabismus is overexpressed

Over expression of Strabismus leads to a loss of Diego asymmetry.