Localisation of Dishevelled in different mutant backgrounds

Dishevelled::GFP in a flamingo^E45/E59 clone

Loss of flamingo leads to a large reduction of Dishevelled from the cell junctions, and a loss of asymmetry.

Flamingo is required for junctional asymmetric localisation of Dishevelled

Confocal images of pupal wings at 30 hours APF. Anterior to the left.

Dishevelled::GFP reduced at junctions in absence of Flamingo.

Reference

Unipolar membrane association of Dishevelled mediates Frizzled planar cell polarity signaling. Jeffery D. Axelrod, 2001

• Pubmed reference

Dishevelled::GFP in a frizzled^J22 mutant background

Loss of frizzled disrupts Dishevelled junctional localisation and asymmetry.

Dishevelled::GFP reduced at junctions and not asymmetric in absence of Frizzled.

Confocal images of pupal wings at about 30 hours. Distal is rightwards and anterior is upwards.

Reference

Unipolar membrane association of Dishevelled mediates Frizzled planar cell polarity signaling. Jeffery D. Axelrod, 2001

• Flybase reference

• Pubmed reference

Dishevelled in a Strabismus⁶ mutant background

Loss of Strabismus leads to a large reduction in Dishevelled at the membrane.

Loss of Strabismus disrupts Dishevelled astymmetry and localisation at the junctions.

Strabismus is required for asymmetric localisation and accumulation of Dishevelled.

The image shows a loss-of-function clone; mutant tissue is indicated by the absence of lacZ staining (red, left panels).

Reference

Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning. Rebecca Bastock, 2003.

• Flybase reference

• Pubmed reference

Dishevelled in a diego mutant background

Loss of clear junctional localisation of Dishevelled in the absence of Diego.

Diego is required for the localisation of Dishevelled and the formation of puncta.

Dishevelled localisation (Dishevelled::GFP in green, ubiquitously expressed from its endogenous promoter) in wing imaginal discs; projection of horizontal optical sections in the apical region.

enGal4/ UAS-Diego; Dishevelled::GFP/+ (at 29oC). Diego is overexpressed in the posterior compartment (anti-Diego in red). Dishevelled::GFP enriched apically in the posterior compartment where Diego is overexpressed (compare to Anterior compartment, left side of panels).

Reference

Jun Wu, Andreas Jenny, Ivana Mirkovic, Marek Mlodzik, Mechanisms of Development, 2008.

Dishevelled in a prickle mutant background

Dishevelled levels not altered, but asymmetry is lost, in the absence of pk-sple.

In the absence of Prickle, Strabismus puncta are smaller and Strabismus asymmetry is lost.

pk-sple13 mutant. Prickle is required for asymmetric localisation and accumulation of polarity proteins.

Confocal images of pupal wings at about 28 hours. Distal is rightwards and anterior is upwards. Loss-of-function clones, mutant tissue indicated by absence of lacZ staining (red, left panel). 30APF pupl winng imunostained for Dishevelled

Reference

Prickle Mediates Feedback Amplification to Generate Asymmetric Planar Cell Polarity Signaling. David Tree et al., 2002.

• Flybase reference

• Pubmed reference

Dishevelled in a >>Prickle over-expression background

An increase in membrane localisation of Dishevelled in the pupal wing.

Overexpression of Prickle leads to a loss of Dishevelled::GFP asymmetry but an increase in large puncta.

This image is near the anterior-posterior boundary of 30 hr APF pupal wing overexpressing UAS-Pk in the engrailed (posterior) domain (en-Gal4). Posterior is denoted by the green bar.

Reference

Prickle Mediates Feedback Amplification to Generate Asymmetric Planar Cell Polarity Signaling. David Tree et al., 2002.

• Pubmed reference

Over expression of >>Strabismus leads to a loss of Dishevelled asymmetry

Reference

Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning. Rebecca Bastock, 2003.

• Flybase reference

• Pubmed reference

Over expression of >>Diego leads to a loss of Dishevelled asymmetry

Reference

Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning. Rebecca Bastock, 2003.

• Flybase reference

• Pubmed reference

Over expression of Flamingo leads to an increase in Dishevelled at the junctions

Loss of asymmetry and an increase in the amount of Dishevelled at the junctions.

Over expression of Flamingo leads to a loss of Dishevelled asymmetry and an increases at the junctions.

This result suggests that along with Frizzled, Dishevelled is preferentially recruited to the junctions in the presence of more Flamingo.

Anti-Dishevelled in Act-GAL4/UAS-Flamingo overexpression clone.

Reference

Differential stability of flamingo protein complexes underlies the establishment of planar polarity. H Strutt and D Strutt, 2008.

• Pubmed reference

Dishevelled in an Frizzled over-expression background

Dsh localisation when UAS-Fz is overexpressed.

Over expression of Frizzled leads an accumulation of Dishevelled at the membrane.

(J) Pk and Dsh colocalize in ptc-GAL4/UAS-fz wings (Pk, red; Dsh:GFP, green).

Reference

Prickle Mediates Feedback Amplification to Generate Asymmetric Planar Cell Polarity Signaling. David Tree et al., 2002.

• Flybase reference

• Pubmed reference